Emma Hayes has made four changes to her side from the big 8-0 win over Leicester at the weekend.
Returning to the starting 11 tonight in Spain are defenders Jess Carter and Kadeisha Buchanan, midfielder Erin Cuthbert, who was a scorer in the home meeting with Real, and Lauren James joins the attack. Carter makes her 100th Chelsea appearance.
Making way are Eve Perisset, Niamh Charles, Johanna Rytting Kaneryd and Fran Kirby.
Sam Kerr again leads the attack. Sophie Ingle, the other scorer in 2-0 win over the Madrid side at Kingsmeadow, starts and Maren Mjelde is back among the subs.
Real Madrid name Scotland international Caroline Weir in their starting line-up.
Chelsea Berger; Carter, Buchanan, Bright, Eriksson (c), Cuthbert, Ingle, James, Fleming, Reiten, Kerr.
Subs Musovic, Orman, Abdullina, Charles, Perisset, Mjelde, Nouwen, Cankovic, Rytting Kaneryd, Svitkova, Kirby, England.
Real Madrid Rodriguez; Andres (c), Sousa, Galvez, Carmona, Weir, Toletti, Zornoza, Del Castillo, Feller, Gonzalez.
Subs Gerard, Fuente, Kenti Robles, Abelleira, Claudia Florentino, Nahikari Garcia, Lucia Rodriguez, Moller, Svava, Olofsson, Maite Oroz, Camacho.
The game will be live streamed on The 5th Stand and chelseafc.com